stranded oligonucleotide hot probes Search Results


double-stranded oligonucleotide probes ap-1 nuclear factor κb (nf-κb  (Promega)
90
Promega double-stranded oligonucleotide probes ap-1 nuclear factor κb (nf-κb
PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay <t>using</t> <t>AP-1</t> (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.
Double Stranded Oligonucleotide Probes Ap 1 Nuclear Factor κb (Nf κb, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stranded+oligonucleotide+hot+probes/double+stranded+oligonucleotide+probes+ap+1+nuclear+factor+%CE%BAb++nf+%CE%BAb/pmc04576286-111-4-13
Average 90 stars, based on 1 article reviews
double-stranded oligonucleotide probes ap-1 nuclear factor κb (nf-κb - by Bioz Stars, 2026-11
90/100 stars
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doublestranded oligonucleotide probe specific for the b site  (Geneka Biotechnology Inc)
90
Geneka Biotechnology Inc doublestranded oligonucleotide probe specific for the b site
PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay <t>using</t> <t>AP-1</t> (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.
Doublestranded Oligonucleotide Probe Specific For The B Site, supplied by Geneka Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stranded+oligonucleotide+hot+probes/32p+end+labeled++double+stranded+oligonucleotide+probe+specific+b+site/10__1164_slash_rccm__200603___341oc-79-46-60
Average 90 stars, based on 1 article reviews
doublestranded oligonucleotide probe specific for the b site - by Bioz Stars, 2026-11
90/100 stars
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double-stranded c/ebp wild type and mutant oligonucleotides  (Geneka Biotechnology Inc)
90
Geneka Biotechnology Inc double-stranded c/ebp wild type and mutant oligonucleotides
PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay <t>using</t> <t>AP-1</t> (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.
Double Stranded C/Ebp Wild Type And Mutant Oligonucleotides, supplied by Geneka Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stranded+oligonucleotide+hot+probes/double+stranded+oligonucleotide+probe/10__1074_slash_jbc__m202653200-129-5-10
Average 90 stars, based on 1 article reviews
double-stranded c/ebp wild type and mutant oligonucleotides - by Bioz Stars, 2026-11
90/100 stars
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radiolabeled, double-stranded oligonucleotide probes sp-1  (Promega)
90
Promega radiolabeled, double-stranded oligonucleotide probes sp-1
PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay <t>using</t> <t>AP-1</t> (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.
Radiolabeled, Double Stranded Oligonucleotide Probes Sp 1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stranded+oligonucleotide+hot+probes/radiolabeled++double+stranded+oligonucleotide+probes+sp+1/10__1074_slash_jbc__m103166200-100-33-39
Average 90 stars, based on 1 article reviews
radiolabeled, double-stranded oligonucleotide probes sp-1 - by Bioz Stars, 2026-11
90/100 stars
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double-stranded oligonucleotide probe  (Promega)
90
Promega double-stranded oligonucleotide probe
PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay <t>using</t> <t>AP-1</t> (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.
Double Stranded Oligonucleotide Probe, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stranded+oligonucleotide+hot+probes/double+stranded+oligonucleotide+probe/pm12388372-107-22-39
Average 90 stars, based on 1 article reviews
double-stranded oligonucleotide probe - by Bioz Stars, 2026-11
90/100 stars
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double-stranded sp1 consensus oligonucleotide probe  (Promega)
90
Promega double-stranded sp1 consensus oligonucleotide probe
PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay <t>using</t> <t>AP-1</t> (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.
Double Stranded Sp1 Consensus Oligonucleotide Probe, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stranded+oligonucleotide+hot+probes/double+stranded+sp1+consensus+oligonucleotide+probe/pm08662878-72-3-10
Average 90 stars, based on 1 article reviews
double-stranded sp1 consensus oligonucleotide probe - by Bioz Stars, 2026-11
90/100 stars
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21-mer corresponding to the ap-1 consensus sequence  (Promega)
90
Promega 21-mer corresponding to the ap-1 consensus sequence
PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay <t>using</t> <t>AP-1</t> (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.
21 Mer Corresponding To The Ap 1 Consensus Sequence, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stranded+oligonucleotide+hot+probes/21+mer+double+stranded+synthetic+oligonucleotide+probe/pmc03561261-62-14-23
Average 90 stars, based on 1 article reviews
21-mer corresponding to the ap-1 consensus sequence - by Bioz Stars, 2026-11
90/100 stars
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the double-stranded dna oligonucleotide probes for the gel shift experiments  (Promega)
90
Promega the double-stranded dna oligonucleotide probes for the gel shift experiments
PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay <t>using</t> <t>AP-1</t> (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.
The Double Stranded Dna Oligonucleotide Probes For The Gel Shift Experiments, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stranded+oligonucleotide+hot+probes/the+double+stranded+dna+oligonucleotide+probes+for+the+gel+shift+experiments/10__1128_slash_mcb__17__5__2550-63-2-18
Average 90 stars, based on 1 article reviews
the double-stranded dna oligonucleotide probes for the gel shift experiments - by Bioz Stars, 2026-11
90/100 stars
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double-stranded oligonucleotide probes containing the mouse immunoglobulin kappa (igκ) light-chain nf-κb consensus site  (Promega)
90
Promega double-stranded oligonucleotide probes containing the mouse immunoglobulin kappa (igκ) light-chain nf-κb consensus site
PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay <t>using</t> <t>AP-1</t> (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.
Double Stranded Oligonucleotide Probes Containing The Mouse Immunoglobulin Kappa (Igκ) Light Chain Nf κb Consensus Site, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stranded+oligonucleotide+hot+probes/double+stranded+oligonucleotide+probes+containing+the+mouse+immunoglobulin+kappa++ig%CE%BA++light+chain+nf+%CE%BAb+consensus+site/pmc11158067-71-0-16
Average 90 stars, based on 1 article reviews
double-stranded oligonucleotide probes containing the mouse immunoglobulin kappa (igκ) light-chain nf-κb consensus site - by Bioz Stars, 2026-11
90/100 stars
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32 p-labeled nf-κb p50 oligonucleotides, 5′-gcc atg ggg gga tcc ccg aag tcc-3′  (Geneka Biotechnology Inc)
90
Geneka Biotechnology Inc 32 p-labeled nf-κb p50 oligonucleotides, 5′-gcc atg ggg gga tcc ccg aag tcc-3′
PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay <t>using</t> <t>AP-1</t> (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.
32 P Labeled Nf κb P50 Oligonucleotides, 5′ Gcc Atg Ggg Gga Tcc Ccg Aag Tcc 3′, supplied by Geneka Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stranded+oligonucleotide+hot+probes/double+stranded+oligonucleotide+probe+specific+for+the+%CE%BAb+site/pmc02880493-127-13-24
Average 90 stars, based on 1 article reviews
32 p-labeled nf-κb p50 oligonucleotides, 5′-gcc atg ggg gga tcc ccg aag tcc-3′ - by Bioz Stars, 2026-11
90/100 stars
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double-stranded oligonucleotide probe containing the immunoglobulin/hiv- κ b enhancer sequence  (Promega)
90
Promega double-stranded oligonucleotide probe containing the immunoglobulin/hiv- κ b enhancer sequence
PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay <t>using</t> <t>AP-1</t> (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.
Double Stranded Oligonucleotide Probe Containing The Immunoglobulin/Hiv κ B Enhancer Sequence, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stranded+oligonucleotide+hot+probes/double+stranded+oligonucleotide+probe+containing+the+immunoglobulin+hiv++%CE%BA+b+enhancer+sequence/pmc03836592-66-15-24
Average 90 stars, based on 1 article reviews
double-stranded oligonucleotide probe containing the immunoglobulin/hiv- κ b enhancer sequence - by Bioz Stars, 2026-11
90/100 stars
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22-mer double-stranded oligonucleotide probe containing a consensus-binding sequence for nf- b  (Promega)
90
Promega 22-mer double-stranded oligonucleotide probe containing a consensus-binding sequence for nf- b
PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay <t>using</t> <t>AP-1</t> (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.
22 Mer Double Stranded Oligonucleotide Probe Containing A Consensus Binding Sequence For Nf B, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stranded+oligonucleotide+hot+probes/22+mer+double+stranded+oligonucleotide+probe+containing+a+consensus+binding+sequence+for+nf++b++5++agt+tga+ggg+gac+ttt+ccc+agg+c+3/pm12960255-74-10-22
Average 90 stars, based on 1 article reviews
22-mer double-stranded oligonucleotide probe containing a consensus-binding sequence for nf- b - by Bioz Stars, 2026-11
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Image Search Results


PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay using AP-1 (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.

Journal: World Journal of Gastroenterology : WJG

Article Title: 4-hydroxy-2, 3-nonenal activates activator protein-1 and mitogen-activated protein kinases in rat pancreatic stellate cells

doi: 10.3748/wjg.v10.i16.2344

Figure Lengend Snippet: PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay using AP-1 (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.

Article Snippet: Double-stranded oligonucleotide probes for AP-1 and nuclear factor κB (NF-κB) were purchased from Promega (Madison WI).

Techniques: Mobility Shift

HNE increased AP-1, but not NF-κB, -dependent transcriptional activity. A: PSCs were transfected with the lu-ciferase vectors (2 X AP-1 or 2 X NF-κB) along with pRL-TK vector as an internal control. After 24 h, the transfected cells were treated with IL-1β (at 2 ng/mL) or HNE (at 1 μmol/L ). After another 24-h incubation, intracellular luciferase activities were determined. The data represent mean mean ± SD, calcu-lated from three independent experiments as fold induction compared with the activity observed in control (serum-free medium only, “Con”). bP < 0.01 vs control. RLU: relative light units. B: PSCs were treated with IL-1β (at 2 ng/mL) or HNE (at 1 μmol/L) in serum-free medium for the indicated times. Total cell lysates were prepared, and the level of IκB-α was deter-mined by Western blotting.

Journal: World Journal of Gastroenterology : WJG

Article Title: 4-hydroxy-2, 3-nonenal activates activator protein-1 and mitogen-activated protein kinases in rat pancreatic stellate cells

doi: 10.3748/wjg.v10.i16.2344

Figure Lengend Snippet: HNE increased AP-1, but not NF-κB, -dependent transcriptional activity. A: PSCs were transfected with the lu-ciferase vectors (2 X AP-1 or 2 X NF-κB) along with pRL-TK vector as an internal control. After 24 h, the transfected cells were treated with IL-1β (at 2 ng/mL) or HNE (at 1 μmol/L ). After another 24-h incubation, intracellular luciferase activities were determined. The data represent mean mean ± SD, calcu-lated from three independent experiments as fold induction compared with the activity observed in control (serum-free medium only, “Con”). bP < 0.01 vs control. RLU: relative light units. B: PSCs were treated with IL-1β (at 2 ng/mL) or HNE (at 1 μmol/L) in serum-free medium for the indicated times. Total cell lysates were prepared, and the level of IκB-α was deter-mined by Western blotting.

Article Snippet: Double-stranded oligonucleotide probes for AP-1 and nuclear factor κB (NF-κB) were purchased from Promega (Madison WI).

Techniques: Activity Assay, Transfection, Plasmid Preparation, Incubation, Luciferase, Western Blot